Evolving a Thermostable DNA Polymerase That Amplifies from Highly Damaged Templates | Zendy

Gloeckner Christian | Zendy; Sauter Katharina B. M. | Zendy; Marx Andreas | Zendy

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Evolving a Thermostable DNA Polymerase That Amplifies from Highly Damaged Templates

Author(s) -

Gloeckner Christian,

Sauter Katharina B. M.,

Marx Andreas

Publication year - 2007

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200603987

Subject(s) - dna polymerase , polymerase , dna polymerase ii , dna clamp , biology , dna replication , dna damage , dna , dna polymerase mu , dna polymerase delta , dna repair , biochemistry , ultraviolet light , eukaryotic dna replication , chemistry , circular bacterial chromosome , polymerase chain reaction , gene , reverse transcriptase , photochemistry

The foreign lesion : DNAs with damage generated, for example, by UV light are not good targets for standard replicative DNA polymerases. A DNA polymerase has been derived by directed evolution that faithfully amplifies light‐damaged DNA. Interestingly, a single mutation is sufficient for this property (see picture).

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