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Evolving a Thermostable DNA Polymerase That Amplifies from Highly Damaged Templates
Author(s) -
Gloeckner Christian,
Sauter Katharina B. M.,
Marx Andreas
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200603987
Subject(s) - dna polymerase , polymerase , dna polymerase ii , dna clamp , biology , dna replication , dna damage , dna , dna polymerase mu , dna polymerase delta , dna repair , biochemistry , ultraviolet light , eukaryotic dna replication , chemistry , circular bacterial chromosome , polymerase chain reaction , gene , reverse transcriptase , photochemistry
The foreign lesion : DNAs with damage generated, for example, by UV light are not good targets for standard replicative DNA polymerases. A DNA polymerase has been derived by directed evolution that faithfully amplifies light‐damaged DNA. Interestingly, a single mutation is sufficient for this property (see picture).