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Selective Extraction of Peptides from Human Plasma by Highly Ordered Mesoporous Silica Particles for Peptidome Analysis
Author(s) -
Tian Ruijun,
Zhang He,
Ye Mingliang,
Jiang Xiaogang,
Hu Lianghai,
Li Xin,
Bao Xinhe,
Zou Hanfa
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200603917
Subject(s) - ultrafiltration (renal) , mesoporous silica , extraction (chemistry) , human plasma , chromatography , peptide , chemistry , mesoporous material , adsorption , chemical engineering , materials science , biophysics , nanotechnology , biochemistry , organic chemistry , biology , engineering , catalysis
Pores for effect : Silica particles with a pore size of 20.5 Å (see TEM image) are effective for enriching peptides in human plasma over a wide range of molecular weights from 1 to 12 kDa, while repelling most other plasma proteins outside. The pore structure of the material makes it superior for peptide enrichment compared to adsorbent‐ and ultrafiltration‐based methods.