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One‐Step Homogeneous Detection of DNA Hybridization with Gold Nanoparticle Probes by Using a Linear Light‐Scattering Technique
Author(s) -
Du BaoAn,
Li ZhengPing,
Liu ChengHui
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200603331
Subject(s) - colloidal gold , oligonucleotide , scattering , homogeneous , dna , light scattering , nanoparticle , dna–dna hybridization , materials science , nanotechnology , optics , chemistry , analytical chemistry (journal) , microbiology and biotechnology , physics , biology , chromatography , biochemistry , thermodynamics
Matchmaker, matchmaker : A gold‐nanoparticle‐based light‐scattering assay has been developed for sensitive detection of DNA hybridization. The assay relies on the enhancement of light scattering that originates from the aggregation of oligonucleotide‐functionalized gold nanoparticles directed by the target DNA. The assay also exhibits a high degree of discrimination between perfectly matched target DNA and targets with single base‐pair mismatches.