The Diagnostic Substrate Bicyclohexane Reveals a Radical Mechanism for Bacterial Cytochrome P450 in Whole Cells | Zendy

Austin Rachel N. | Zendy; Deng Dayi | Zendy; Jiang Yongying | Zendy; Luddy Kate | Zendy; van Beilen Jan B. | Zendy; Ortiz de Montellano Paul R. | Zendy; Groves John T. | Zendy

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The Diagnostic Substrate Bicyclohexane Reveals a Radical Mechanism for Bacterial Cytochrome P450 in Whole Cells

Author(s) -

Austin Rachel N.,

Deng Dayi,

Jiang Yongying,

Luddy Kate,

van Beilen Jan B.,

Ortiz de Montellano Paul R.,

Groves John T.

Publication year - 2006

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200603282

Subject(s) - oxidizing agent , substrate (aquarium) , cytochrome , chemistry , alkane , substrate specificity , mechanism (biology) , stereochemistry , combinatorial chemistry , biochemistry , organic chemistry , catalysis , ecology , biology , enzyme , philosophy , epistemology

On home ground : The reaction mechanisms of bacterial alkane‐oxidizing cytochrome P450s were determined in their native environment using a novel diagnostic substrate probe, bicyclohexane, in whole cells and cell‐free extracts (see picture). Purified P450cam also oxidizes bicyclohexane. Clear evidence for a substrate‐based radical with a lifetime of 75–250 ps was obtained.

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