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The Diagnostic Substrate Bicyclohexane Reveals a Radical Mechanism for Bacterial Cytochrome P450 in Whole Cells
Author(s) -
Austin Rachel N.,
Deng Dayi,
Jiang Yongying,
Luddy Kate,
van Beilen Jan B.,
Ortiz de Montellano Paul R.,
Groves John T.
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200603282
Subject(s) - oxidizing agent , substrate (aquarium) , cytochrome , chemistry , alkane , substrate specificity , mechanism (biology) , stereochemistry , combinatorial chemistry , biochemistry , organic chemistry , catalysis , ecology , biology , enzyme , philosophy , epistemology
On home ground : The reaction mechanisms of bacterial alkane‐oxidizing cytochrome P450s were determined in their native environment using a novel diagnostic substrate probe, bicyclohexane, in whole cells and cell‐free extracts (see picture). Purified P450cam also oxidizes bicyclohexane. Clear evidence for a substrate‐based radical with a lifetime of 75–250 ps was obtained.