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Substrate Mimicry in an Activity‐Based Probe That Targets the Nitrilase Family of Enzymes
Author(s) -
Barglow Katherine T.,
Cravatt Benjamin F.
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200603187
Subject(s) - nitrilase , dipeptide , substrate (aquarium) , enzyme , chemistry , mimicry , substrate specificity , biochemistry , stereochemistry , combinatorial chemistry , amino acid , biology , ecology
Up and at it : A set of activity‐based proteomics probes containing a dipeptide α‐chloroacetamide scaffold that targets the nitrilase family of enzymes is described. One member of the nitrilase class, ureidopropionase‐β, was found to react selectively with a single probe that mimics the enzyme's endogenous substrate N ‐carbamoyl β‐alanine.