Premium
On the Acceptor Substrate of C‐Glycosyltransferase UrdGT2: Three Prejadomycin C‐Glycosides from an Engineered Mutant of Streptomyces globisporus 1912 Δ lndE ( urdGT2 )
Author(s) -
Baig Irfan,
Kharel Madan,
Kobylyanskyy Anton,
Zhu Lili,
Rebets Yuriy,
Ostash Bohdan,
Luzhetskyy Andriy,
Bechthold Andreas,
Fedorenko Victor A.,
Rohr Jürgen
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200603176
Subject(s) - glycosyltransferase , mutant , acceptor , glycosylation , substrate (aquarium) , gene , chemistry , stereochemistry , biochemistry , biology , physics , ecology , condensed matter physics
The transfer of the gene urdGT2 , which encodes a C‐glycosyltransferase in the urdamycin pathway, into the lnd ‐minus mutant of the landomycin ( lnd ) biosynthetic pathway indicates the difference in the first glycosylation step in the urdamycin and landomycin pathways. It also reveals indirectly the acceptor substrate of UrdGT2 (see scheme) and gives evidence of greatly relaxed substrate specificity of all lnd glycosyltransferases except LndGT2.