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Iterative Saturation Mutagenesis on the Basis of B Factors as a Strategy for Increasing Protein Thermostability
Author(s) -
Reetz Manfred T.,
Carballeira José D.,
Vogel Andreas
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200602795
Subject(s) - thermostability , saturated mutagenesis , saturation (graph theory) , mutagenesis , directed evolution , protein engineering , computational biology , chemistry , biology , biological system , biochemistry , mutation , mathematics , enzyme , combinatorics , gene , mutant
Efficiency counts: A new method of directed evolution for increasing the thermostability of proteins saves time and effort. In an illustrative study, iterative cycles of saturation mutagenesis were performed at those sites in a lipase that display high B factors.