Iterative Saturation Mutagenesis on the Basis of B Factors as a Strategy for Increasing Protein Thermostability | Zendy

Reetz Manfred T. | Zendy; Carballeira José D. | Zendy; Vogel Andreas | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Iterative Saturation Mutagenesis on the Basis of B Factors as a Strategy for Increasing Protein Thermostability

Author(s) -

Reetz Manfred T.,

Carballeira José D.,

Vogel Andreas

Publication year - 2006

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200602795

Subject(s) - thermostability , saturated mutagenesis , saturation (graph theory) , mutagenesis , directed evolution , protein engineering , computational biology , chemistry , biology , biological system , biochemistry , mutation , mathematics , enzyme , combinatorics , gene , mutant

Efficiency counts: A new method of directed evolution for increasing the thermostability of proteins saves time and effort. In an illustrative study, iterative cycles of saturation mutagenesis were performed at those sites in a lipase that display high B factors.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research