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Evolving Thermostable Reverse Transcriptase Activity in a DNA Polymerase Scaffold
Author(s) -
Sauter Katharina B. M.,
Marx Andreas
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200602772
Subject(s) - reverse transcriptase , dna polymerase , polymerase , dna , chemistry , dna clamp , scaffold , rna directed dna polymerase , enzyme , microbiology and biotechnology , rna , biology , biochemistry , gene , medicine , biomedical engineering
Active mutants : Thermostable DNA polymerases normally exhibit no significant intrinsic activities to reverse transcribe RNA into DNA. We show that enzymes with significantly increased reverse transcription ability can be generated by directed evolution (see picture).