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Isotope‐Labeled Photoaffinity Reagents and Mass Spectrometry To Identify Protein–Ligand Interactions
Author(s) -
Sinz Andrea
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200602549
Subject(s) - photoaffinity labeling , reagent , chemistry , mass spectrometry , ligand (biochemistry) , combinatorial chemistry , isotope , tandem mass tag , chromatography , stable isotope ratio , biochemistry , binding site , proteomics , quantitative proteomics , organic chemistry , receptor , physics , quantum mechanics , gene
The target proteins of bioactive low‐molecular‐weight ligands can be discovered by the use of multifunctional photoaffinity labels in combination with mass spectrometry. The reagents contain a biotin label for affinity purification of the protein–ligand complex and a stable isotope label to facilitate its mass spectrometric identification (see scheme).
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