Absolute and Accurate Quantification of Protein Phosphorylation by Using an Elemental Phosphorus Standard and Element Mass Spectrometry | Zendy

Pereira Navaza A. | Zendy; Ruiz Encinar J. | Zendy; SanzMedel A. | Zendy

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Absolute and Accurate Quantification of Protein Phosphorylation by Using an Elemental Phosphorus Standard and Element Mass Spectrometry

Author(s) -

Pereira Navaza A.,

Ruiz Encinar J.,

SanzMedel A.

Publication year - 2007

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200602517

Subject(s) - phosphopeptide , phosphoproteomics , mass spectrometry , chemistry , phosphorus , phosphorylation , computer science , chromatography , analytical chemistry (journal) , database , information retrieval , protein phosphorylation , biochemistry , organic chemistry , protein kinase a

The simpler the better : The combination of element mass spectrometry under optimized conditions and an elemental phosphorus standard provides a generic approach for absolute quantitative phosphoproteomics. This approach allows for simultaneous and reliable quantification of each individual phosphopeptide (corresponding to the different phosphorylation sites) present in a tryptic digest of a protein mixture.

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