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Absolute and Accurate Quantification of Protein Phosphorylation by Using an Elemental Phosphorus Standard and Element Mass Spectrometry
Author(s) -
Pereira Navaza A.,
Ruiz Encinar J.,
SanzMedel A.
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200602517
Subject(s) - phosphopeptide , phosphoproteomics , mass spectrometry , chemistry , phosphorus , phosphorylation , computer science , chromatography , analytical chemistry (journal) , database , information retrieval , protein phosphorylation , biochemistry , organic chemistry , protein kinase a
The simpler the better : The combination of element mass spectrometry under optimized conditions and an elemental phosphorus standard provides a generic approach for absolute quantitative phosphoproteomics. This approach allows for simultaneous and reliable quantification of each individual phosphopeptide (corresponding to the different phosphorylation sites) present in a tryptic digest of a protein mixture.