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Photoswitching of the Fluorescent Protein asFP595: Mechanism, Proton Pathways, and Absorption Spectra
Author(s) -
Schäfer Lars V.,
Groenhof Gerrit,
Klingen Astrid R.,
Ullmann G. Matthias,
BoggioPasqua Martial,
Robb Michael A.,
Grubmüller Helmut
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200602315
Subject(s) - chromophore , isomerization , chemistry , fluorescence , fluorescent protein , proton , molecular switch , cascade , mechanism (biology) , absorption (acoustics) , chemical physics , photochemistry , molecule , green fluorescent protein , physics , organic chemistry , biochemistry , quantum mechanics , chromatography , acoustics , gene , catalysis
Molecular light‐switch : Off–on switching of the fluorescence of the protein asFP595 involves a trans – cis isomerization. Mixed quantum/classical simulations elucidate the spectroscopic properties of asFP595 and give detailed insights into the photoswitching mechanism. The conformational trans – cis switching triggers a proton‐transfer cascade between the chromophore and adjacent amino acids.