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Fluorescence Visualization of Newly Synthesized Proteins in Mammalian Cells
Author(s) -
Beatty Kimberly E.,
Liu Julie C.,
Xie Fang,
Dieterich Daniela C.,
Schuman Erin M.,
Wang Qian,
Tirrell David A.
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200602114
Subject(s) - proteome , complement (music) , visualization , fluorescence , computational biology , character (mathematics) , amino acid , biology , chemistry , microbiology and biotechnology , biochemistry , computer science , data mining , physics , gene , quantum mechanics , complementation , phenotype , geometry , mathematics
Playing tag : Noncanonical amino acid tagging enables the selective fluorescent visualization of newly synthesized proteins in mammalian cells (see the picture). Susceptibility to tagging is determined by the spatial and temporal character of the protein synthesis, thus providing a complement to methods which identify relevant members of the proteome.