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Reversible Binding of Fluorescent Proteins at DNA–Gold Nanoparticles
Author(s) -
Hazarika Pompi,
Kukolka Florian,
Niemeyer Christof M.
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200602049
Subject(s) - dna , conjugate , fluorescence , dissociation (chemistry) , colloidal gold , nanoparticle , chemistry , biophysics , nanotechnology , combinatorial chemistry , computational biology , biochemistry , materials science , biology , physics , organic chemistry , mathematics , optics , mathematical analysis
Out of bounds : The reversible immobilization of a fluorescent DNA–protein conjugate by strand displacement is achieved by specific DNA hybridization reactions (see picture). Binding and dissociation of the protein can be followed by observation of the protein's fluorescence, which is quenched upon binding to gold nanoparticles.