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A Population of Thermostable Reverse Transcriptases Evolved from Thermus aquaticus DNA Polymerase I by Phage Display
Author(s) -
VichierGuerre Sophie,
Ferris Stéphane,
Auberger Nicolas,
Mahiddine Karim,
Jestin JeanLuc
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200601217
Subject(s) - thermus aquaticus , reverse transcriptase , dna polymerase , polymerase , population , biology , dna , enzyme , phage display , genetics , microbiology and biotechnology , computational biology , polymerase chain reaction , gene , biochemistry , demography , sociology , antibody
Running in reverse : A set of enzymes selected according to their RNA‐dependent DNA polymerase activity from a library of more than 10 7 mutant enzymes has been characterized. The product was specifically bound to catalytically active proteins displayed on filamentous bacteriophage. The selected variants have a catalytic efficiency for reverse transcription that is two orders of magnitude higher.