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High Thermal Stability of 5′‐5′‐Linked Alternate Hoogsteen Triplexes at Physiological pH
Author(s) -
Filichev Vyacheslav V.,
Nielsen Mads C.,
Bomholt Niels,
Jessen Carsten H.,
Pedersen Erik B.
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200601127
Subject(s) - dna , thermal stability , melting temperature , linker , chemistry , triple helix , thermal , double stranded , biophysics , stereochemistry , thermodynamics , computer science , biochemistry , physics , biology , materials science , organic chemistry , programming language , composite material
DNA glue : A new alternate‐strand linker (shown in green) has been synthesized that can connect two short DNA sequences (5‐ and 9‐mers, yellow) and bind to double‐stranded DNA through major‐groove interactions at pH 7.2 (see model). The resulting triple helices have high thermal stabilities as shown by melting temperatures exceeding 37 °C at physiological pH values.