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Turning Inhibitors into Activators: A Hammerhead Ribozyme Controlled by a Guanine Quadruplex
Author(s) -
Wieland Markus,
Hartig Jörg S.
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200600909
Subject(s) - ribozyme , guanosine , vs ribozyme , hammerhead ribozyme , g quadruplex , computational biology , chemistry , nucleic acid , modular design , biochemistry , computer science , stereochemistry , biology , rna , dna , gene , programming language
Modular design of functional RNAs can be used to tailor molecular properties. Guanosine‐rich sequences were introduced as switching devices for functional nucleic acids. TMPyP4 (see picture) was identified as the strongest ribozyme inhibitor known to date. When quadruplex‐forming, G‐rich sequences were attached to the ribozyme, the TMPyP4‐mediated response was inverted from inhibition to activation of the ribozyme reaction.