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Four‐Color, Enzyme‐Free Interrogation of DNA Sequences with Chemically Activated, 3′‐Fluorophore‐Labeled Nucleotides
Author(s) -
Griesang Niels,
Gießler Kerstin,
Lommel Tanja,
Richert Clemens
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200600804
Subject(s) - fluorophore , nucleotide , primer (cosmetics) , primer extension , dna , enzyme , nucleoside , chemistry , biochemistry , microbiology and biotechnology , biology , base sequence , fluorescence , physics , gene , optics , organic chemistry
Color reveals base : The enzymatic template‐directed elongation of primers with fluorophore‐labeled nucleoside triphosphates is the most frequently used reaction for determining DNA sequences. Primer extension followed by optical read out can now be achieved without enzymes.