SNP Genotyping by Using Photochemical Ligation | Zendy

Ogasawara Shinzi | Zendy; Fujimoto Kenzo | Zendy

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SNP Genotyping by Using Photochemical Ligation

Author(s) -

Ogasawara Shinzi,

Fujimoto Kenzo

Publication year - 2006

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200600790

Subject(s) - oligonucleotide , snp genotyping , genotyping , molecular inversion probe , single nucleotide polymorphism , dna microarray , context (archaeology) , hybridization probe , genetics , multiplex ligation dependent probe amplification , oligomer restriction , biology , dna , microbiology and biotechnology , computational biology , genotype , gene , gene expression , paleontology , exon

A chip for single mismatches : Results obtained with a photochemical‐ligation‐based DNA chip for enhanced discrimination of single‐nucleotide polymorphisms (see scheme) show that the reaction takes place over a period of hours, with the 50 % stage reached within 10 minutes. A single mismatch in the target sequence yielded extremely little ligated product (a rate 10 3 ‐fold lower than for the complementary case).

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