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Site‐Specific Protein Modification through Cu I ‐Catalyzed 1,2,3‐Triazole Formation and Its Implementation in Protein Microarray Fabrication
Author(s) -
Lin PoChiao,
Ueng ShauHua,
Tseng MeiChun,
Ko JiaLing,
Huang KuoTing,
Yu ShengChieh,
Adak Avijit Kumar,
Chen YuJu,
Lin ChunCheng
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200600756
Subject(s) - chemistry , catalysis , click chemistry , triazole , combinatorial chemistry , biochemistry , organic chemistry
Out of site : A protein expression system was combined with Cu I ‐catalyzed 1,2,3‐triazole formation to modify a target protein at its C terminus. The immobilized core protein can be modified by diverse small molecules in a site‐specific manner (see picture). Protein conjugation with a diazido linker gives a homodimeric protein, and microarrays can be fabricated by site‐specific covalent binding to functionalized surfaces.