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Mixed Isotope Photoaffinity Reagents for Identification of Small‐Molecule Targets by Mass Spectrometry
Author(s) -
Lamos Shane M.,
Krusemark Casey J.,
McGee Christopher J.,
Scalf Mark,
Smith Lloyd M.,
Belshaw Peter J.
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200600743
Subject(s) - photoaffinity labeling , chemistry , mass spectrometry , deuterium , reagent , identification (biology) , isotope , chromatography , combinatorial chemistry , organic chemistry , biochemistry , binding site , quantum mechanics , physics , botany , biology
Twin peaks : A facile and economic route has been developed for the incorporation of deuterated benzophenones (a widely used photoaffinity tag) into probes. Attachment of these mixed‐isotope labels to the immunosuppressive drug CsA facilitated the mass spectrometric identification of a target of CsA (see picture) from a mixture of proteins.