Premium
Reducing Product Inhibition in DNA‐Template‐Controlled Ligation Reactions
Author(s) -
Dose Christian,
Ficht Simon,
Seitz Oliver
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200600464
Subject(s) - ligation , native chemical ligation , dna , template , chemistry , flexibility (engineering) , zipper , chemical ligation , proximity ligation assay , combinatorial chemistry , biophysics , computer science , materials science , biology , nanotechnology , microbiology and biotechnology , biochemistry , chemical synthesis , algorithm , mathematics , statistics , in vitro , receptor
Loosening the zipper : A ligation–rearrangement reaction sequence is the key to signal amplification in chemical DNA‐templated ligation reactions. The rearrangement increases the flexibility of the ligation intermediate, thereby reducing the affinity of the rearranged product for the DNA template. A fluorescence‐resonant‐energy‐transfer technique is used for convenient and product‐specific real‐time detection of product signals.