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A Genetically Encoded Photocaged Tyrosine
Author(s) -
Deiters Alexander,
Groff Dan,
Ryu Youngha,
Xie Jianming,
Schultz Peter G.
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200600264
Subject(s) - tyrosine , chemistry , enzyme , biochemistry , in vitro , transcription (linguistics) , transduction (biophysics) , biology , microbiology and biotechnology , philosophy , linguistics
Activating proteins with light : A photocaged tyrosine was genetically encoded in E. coli in response to the amber codon TAG. Substitution of Tyr 503 in the active site of β‐galactosidase allowed photoactivation of this enzyme in vitro or directly in bacteria with 360‐nm light. This method should allow photoregulation of the activity of a variety of biological processes including transcription, signal transduction, and cellular trafficking.