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Biocatalytic Evolution of a Biocatalyst Marker: Towards the Ultrasensitive Detection of Immunocomplexes and DNA Analysis
Author(s) -
Shlyahovsky Bella,
Pavlov Valeri,
Kaganovsky Lubov,
Willner Itamar
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200600073
Subject(s) - bovine serum albumin , chemistry , recombinant dna , microbiology and biotechnology , thrombin , dna , substrate (aquarium) , enzyme , chromatography , multiplex , telomerase , biochemistry , fluorescence , biology , gene , immunology , platelet , ecology , bioinformatics , physics , quantum mechanics
Sensitive ELISA : A new enzyme‐labeled immunosorbant assay (ELISA) protocol has been developed that makes use of two enzymatic reactions to detect antibody–antigen interactions: 1) the conversion of prothrombin to thrombin by ecarin and 2) the hydrolysis of a nonfluorescent substrate 1 to a fluorescent product 2 by thrombin. The system was used to detect bovine serum albumin, telomerase, and DNA with high sensitivity.