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Solid‐Phase Enzyme Activity Assay Utilizing an Entrapped Fluorescence‐Signaling DNA Aptamer
Author(s) -
Rupcich Nicholas,
Nutiu Razvan,
Li Yingfu,
Brennan John D.
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200504576
Subject(s) - aptamer , enzyme , fluorescence , substrate (aquarium) , dna , enzyme assay , chemistry , biochemistry , combinatorial chemistry , computational biology , microbiology and biotechnology , biology , physics , ecology , quantum mechanics
Layered enzyme assays : The use of a layered sol‐gel based material allows for spatial separation of coimmobilized enzyme and fluorescent aptamer reporter species. This provides a route for facile assaying of enzyme activity and inhibition in cases where the aptamer signals the presence of the enzyme substrate. This then results in accurate IC 50 determinations.