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Regioselective Glucosylation of Aromatic Compounds: Screening of a Recombinant Glycosyltransferase Library to Identify Biocatalysts
Author(s) -
Weis Markus,
Lim EngKiat,
Bruce Neil,
Bowles Dianna
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200504505
Subject(s) - biocatalysis , regioselectivity , biotransformation , glycosyltransferase , recombinant dna , glucosyltransferase , enzyme , chemistry , biochemistry , computational biology , biology , catalysis , gene , ionic liquid
A novel whole‐cell screen for the identification of new glucosyltransferase (GT) biocatalysts within a recombinant enzyme library was developed. Following biotransformation, levels of D ‐glucose were used as a measure of biocatalysis. Twenty five enzymes that transfer D ‐glucose to trans ‐resveratrol were identified that have the regioselectivity shown in the picture.