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3′‐(2,4‐Dinitrobenzenesulfonyl)‐2′,7′‐dimethylfluorescein as a Fluorescent Probe for Selenols
Author(s) -
Maeda Hatsuo,
Katayama Kohei,
Matsuno Hiromi,
Uno Tadayuki
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200504299
Subject(s) - selenocysteine , thioredoxin reductase , chemistry , fluorescence , thioredoxin , peroxidase , content (measure theory) , thiol , cysteine , combinatorial chemistry , biochemistry , gene , enzyme , physics , mathematical analysis , mathematics , quantum mechanics
Simply the BESThio : The fluorescein derivative BESThio (see scheme) operates as a thiol probe at pH 7.4 and functions as a selenol probe at pH 5.8, and can thus be selectively applied to the rapid quantification of selenocysteine and the determination of the selenocysteine content in selenoproteins such as glutathione peroxidase and thioredoxin reductase with high sensitivity.