Premium
Colorimetric Screening of DNA‐Binding Molecules with Gold Nanoparticle Probes
Author(s) -
Han Min Su,
LyttonJean Abigail K. R.,
Oh ByungKeun,
Heo Jungseok,
Mirkin Chad A.
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200504277
Subject(s) - dna , nanoparticle , molecule , melting temperature , nanotechnology , transition (genetics) , chemistry , colloidal gold , biophysics , crystallography , materials science , gene , biology , biochemistry , organic chemistry , composite material
Nanoparticle assemblies interconnected with DNA can be used to screen for DNA‐binding molecules colorimetrically. The melting temperature of the nanoparticle assembly increases in the presence of strong‐DNA‐binding molecules. Thus, the strength of the DNA binding can be determined by the change in this sharp melting transition, which is observable by a blue‐to‐red color change (see picture).