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Glycosylation of a Neoglycoprotein by Using Glycosynthase and Thioglycoligase Approaches: The Generation of a Thioglycoprotein
Author(s) -
Müllegger Johannes,
Chen Hong Ming,
Warren R. Antony J.,
Withers Stephen G.
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200503900
Subject(s) - glycoprotein , glycosylation , glycoside hydrolase , chemistry , glycosyl , function (biology) , biochemistry , microbiology and biotechnology , enzyme , biology
Remodeling : Glycosylation of a neoglycoprotein was achieved in quantitative yields by using a glycosynthase or a thioglycoligase. The resulting glycoproteins function as good acceptors for glycosyl transferases, and the thioglycoprotein proved to be resistant to glycosidase digestion. The established technology will enable the remodeling of natural glycoproteins to make them more stable against degradation in serum.