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Biosynthesis of the Cyclotide Kalata B1 by Using Protein Splicing
Author(s) -
Kimura Richard H.,
Tran AhnTuyet,
Camarero Julio A.
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200503882
Subject(s) - recombinant dna , rna splicing , biochemistry , alternative splicing , chemistry , biosynthesis , computational biology , biology , gene , exon , rna
The tides are turning : Kalata B1 (KB1) is the prototypical member of an exceptionally stable and resistant family of plant‐derived miniproteins called cyclotides. The biosynthesis of KB1 and a small library of KB1‐based cyclotides (variation occurs in five solvent‐exposed loops) in E. coli has been performed by using recombinant‐DNA expression techniques and protein splicing.