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Visible Sensing of Nucleic Acid Sequences with a Genetically Encodable Unmodified RNA Probe
Author(s) -
Narita Atsushi,
Ogawa Kazumasa,
Sando Shinsuke,
Aoyama Yasuhiro
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200503836
Subject(s) - molecular beacon , rna , dna , rnase p , nucleic acid , computational biology , translation (biology) , gene , sequence (biology) , nucleotide , messenger rna , biology , nucleic acid sequence , microbiology and biotechnology , genetics , chemistry , oligonucleotide
Sensing DNA with RNA : Simple and sensitive DNA sensing was achieved by using an RNA probe which is composed of a molecular‐beacon‐like cis ‐repressing sequence and a gene for the reporter protein. The RNase H activity coupled molecular‐beacon–mRNA system enabled visible sensing of the target nucleotide sequence in a cell‐free translation solution (see picture).