Premium
Autonomous Fueled Mechanical Replication of Nucleic Acid Templates for the Amplified Optical Detection of DNA
Author(s) -
Weizmann Yossi,
Cheglakov Zoya,
Pavlov Valeri,
Willner Itamar
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200503810
Subject(s) - nucleic acid , fluorophore , dna , chemistry , replication (statistics) , bond cleavage , fluorescence , template , combinatorial chemistry , nanotechnology , biochemistry , biology , materials science , physics , catalysis , quantum mechanics , virology
Fuel for a DNA machine : A FokI enzyme/DNA scission machine is triggered by the hybridization of its DNA hairpin structure with a target nucleic acid. The machine is fueled by a secondary fluorophore (F)‐ and quencher (Q)‐functionalized nucleic acid 2 . Scission of 3 leads to replication of the machinery cutter 1 and yields a fluorescent product 6 , which provides an optical output for the machine (see scheme).