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Selective Immobilization of Proteins onto Solid Supports through Split‐Intein‐Mediated Protein Trans‐Splicing
Author(s) -
Kwon Youngeun,
Coleman Matthew A.,
Camarero Julio A.
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200503475
Subject(s) - intein , chemistry , rna splicing , trans splicing , protein splicing , trace (psycholinguistics) , biochemistry , computational biology , combinatorial chemistry , gene , biology , rna , linguistics , philosophy
Without a trace : The immobilization of biologically active proteins onto a solid support through split‐intein‐mediated protein trans‐splicing reactions requires only very dilute samples. Not only is this process traceless but it does not require purification of the protein, thereby making possible the direct immobilization of proteins from complex mixtures such as cell lysates.