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Large‐Scale Synthesis of H‐Antigen Oligosaccharides by Expressing Helicobacter pylori α1,2‐Fucosyltransferase in Metabolically Engineered Escherichia coli Cells
Author(s) -
Drouillard Sophie,
Driguez Hugues,
Samain Eric
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200503427
Subject(s) - fucosyltransferase , escherichia coli , heterologous , lactose , antigen , microbiology and biotechnology , chemistry , biochemistry , recombinant dna , biology , gene , genetics
Sugar production : A microbiological method has been developed for the production of fucosyl α1,2‐linked carbohydrates from lactose. The syntheses of 2′‐fucosyllactose and lacto‐ N ‐neofucopentaose‐1 (see structure), which contain the H‐2 antigen (Fucα‐2Galβ‐4R), were achieved by cultivating Escherichia coli strains that overexpressed the appropriate heterologous genes.