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Diagnostics of Single Base‐Mismatch DNA Hybridization on Gold Nanoparticles by Using the Hyper‐Rayleigh Scattering Technique
Author(s) -
Ray Paresh Chandra
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200503114
Subject(s) - rayleigh scattering , oligonucleotide , dna , dna–dna hybridization , scattering , base pair , colloidal gold , chemistry , microbiology and biotechnology , materials science , nanoparticle , nanotechnology , biophysics , analytical chemistry (journal) , optics , biology , physics , chromatography , biochemistry
Highly sensitive : The hyper‐Rayleigh Scattering (HRS) technique has been used to achieve the ultrasensitive detection of single base‐pair mismatches in oligonucleotide strands without any modification of DNA. A very distinct HRS intensity change is observed after hybridization even at the concentration of 10‐nanomolar probe single‐stranded DNA.