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Label‐Free, Real‐Time Glycosyltransferase Assay Based on a Fluorescent Artificial Chemosensor
Author(s) -
Wongkongkatep Jirarut,
Miyahara Yoshifumi,
Ojida Akio,
Hamachi Itaru
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200503107
Subject(s) - glycosyltransferase , fluorescence , glycosyl , chemistry , combinatorial chemistry , directed evolution , enzyme , biochemistry , chromatography , gene , physics , quantum mechanics , mutant
Reality check : The fluorescent probe 1 is applied to a real‐time glycosyltransferase assay. The fluorescence of 1 increases with the progress of the glycosyl transfer reaction. The high sensitivity and reliable output of the method under multisubstrate enzymatic conditions make it suitable for the high‐throughput screening of glycosyltransferase inhibitors.