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The Transport of Nanosized Gene Carriers Unraveled by Live‐Cell Imaging
Author(s) -
Bausinger Ralf,
von Gersdorff Katharina,
Braeckmans Kevin,
Ogris Manfred,
Wagner Ernst,
Bräuchle Christoph,
Zumbusch Andreas
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200503021
Subject(s) - polyethylenimine , transfection , live cell imaging , biophysics , gene delivery , dna , cell , nanotechnology , chemistry , cytoskeleton , microbiology and biotechnology , nucleus , gene , biology , materials science , biochemistry
Tracking transport : Cationic polyethylenimine nanoparticles serve as nonviral vectors and deliver DNA to the nucleus of cells. Fluorescence live‐cell imaging highlights the interactions between these polyplexes and the cytoskeleton during the subsequent stages of transfection (see picture). The analysis of the underlying transport processes gives new impulse to further chemical‐synthesis approaches.