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Direct Readout of Protein–Protein Interactions by Mass Spectrometry from Protein–DNA Microarrays
Author(s) -
Becker Christian F. W.,
Wacker Ron,
Bouschen Werner,
Seidel Ralf,
Kolaric Branko,
Lang Pascal,
Schroeder Hendrick,
Müller Oliver,
Niemeyer Christof M.,
Spengler Bernhard,
Goody Roger S.,
Engelhard Martin
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200502908
Subject(s) - mass spectrometry , protein microarray , dna microarray , analyte , dna , chemistry , microarray , protein array analysis , proteomics , microbiology and biotechnology , computational biology , biochemistry , biology , chromatography , gene expression , gene
Catching proteins : Proteins from a protein–DNA microarray can be directly detected by using MALDI mass spectrometry. Capture proteins are attached to silicon chips in a highly selective and reversible way by DNA‐directed immobilization (see picture). The analyte proteins bound specifically to their partners on the microarray can be detected from cell lysates.