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Label‐Free Affinity Assays by Rapid Detection of Immune Complexes in Submicrometer Pores
Author(s) -
Uram Jeffrey D.,
Ke Kevin,
Hunt Alan J.,
Mayer Michael
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200502862
Subject(s) - antibody , immune system , chemistry , antigen , enterotoxin , characterization (materials science) , computational biology , nanotechnology , biochemistry , biology , materials science , immunology , escherichia coli , gene
Resistance movement : A submicrometer conical pore was used for the single‐aggregate detection and characterization of immune complexes consisting of proteins such as staphylococcal enterotoxin B and antibodies. The developed assay is rapid, label‐free, requires no immobilization or modification of the antibody or antigen, and can be performed with antibodies or proteins in serum.