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Fluoromorphic Substrates for Fatty Acid Metabolism: Highly Sensitive Probes for Mammalian Medium‐Chain Acyl‐CoA Dehydrogenase
Author(s) -
Froemming Mary K.,
Sames Dalibor
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200502675
Subject(s) - dehydrogenase , biochemistry , beta oxidation , substrate (aquarium) , acyl coa dehydrogenase , fatty acid , metabolism , fatty acid synthesis , fatty acid metabolism , enzyme , acyl coa , substrate specificity , chemistry , biology , ecology
Not fussy : A systematic study revealed considerable substrate tolerance of medium‐chain acyl‐CoA dehydrogenase (MCAD), a member of the β‐oxidation pathway for the degradation of fatty acids. Three fluorogenic reporter substrates were developed, which in turn allowed for selective, sensitive, and continuous monitoring of MCAD activity in tissue homogenates.