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Photobiotin Surface Chemistry Improves Label‐Free Interferometric Sensing of Biochemical Interactions
Author(s) -
Latham Joey C.,
Markov Dmitry A.,
Sørensen Henrik S.,
Bornhop Darryl J.
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200502418
Subject(s) - biotinylation , biosensor , chemistry , nanotechnology , microfluidics , avidin , simple (philosophy) , computer science , combinatorial chemistry , materials science , biochemistry , philosophy , epistemology
A highly sensitive and cost‐effective biosensor : Simple surface chemistry of photobiotin, bound with a layer of avidin, allows convenient immobilization of any biotinylated molecule of interest onto microfluidic channels molded in poly(dimethylsiloxane) (PDMS). The biosensor obtained this way is capable of monitoring biomolecular interactions in label‐free situations and in small (picoliter) volumes.