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Efficient Enzymatic Synthesis of Phosphoroselenoate RNA by Using Adenosine 5′‐(α‐ P ‐Seleno)triphosphate
Author(s) -
Carrasco Nicolas,
CatonWilliams Julianne,
Brandt Gary,
Wang Siming,
Huang Zhen
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200502215
Subject(s) - rna , adenosine triphosphate , enzyme , selenium , chemistry , biochemistry , adenosine , gene , organic chemistry
Se the way to RNA : Adenosine 5′‐(α‐ P ‐seleno)triphosphate (ATPαSe) has been synthesized and efficiently and diastereoselectively incorporated into RNA through enzymatic synthesis (see scheme). The digestion of the phosphoroselenoate RNA with phosphodiesterase is slower than that of the nonmodified RNA. This enzymatic synthesis can be generally applied in the preparation of multimilligram quantities of selenium‐derivatized RNAs for X‐ray crystallography.