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2‐Deoxyribonolactone Lesions in X‐ray‐Irradiated DNA: Quantitative Determination by Catalytic 5‐Methylene‐2‐furanone Release
Author(s) -
Roginskaya Marina,
Razskazovskiy Yuriy,
Bernhard William A.
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200501956
Subject(s) - methylene , catalysis , decomposition , dna , x ray , chemistry , irradiation , high performance liquid chromatography , spermine , combinatorial chemistry , chromatography , biochemistry , organic chemistry , enzyme , physics , nuclear physics , quantum mechanics
Torn genes : DNA tetramers containing the 2‐deoxyribonolactone (dL) lesion have been isolated by HPLC from d(CGCG) and d(pCGCG) films irradiated with X‐rays. Upon treatment with spermine as a catalyst, the dL‐containing tetramers decompose to 5‐methylene‐2‐furanone (5‐MF; see scheme), a characteristic product of dL decomposition. Hence, 5‐MP can be used to quantify dL lesions in DNA.