2‐Deoxyribonolactone Lesions in X‐ray‐Irradiated DNA: Quantitative Determination by Catalytic 5‐Methylene‐2‐furanone Release | Zendy

Roginskaya Marina | Zendy; Razskazovskiy Yuriy | Zendy; Bernhard William A. | Zendy

Premium

2‐Deoxyribonolactone Lesions in X‐ray‐Irradiated DNA: Quantitative Determination by Catalytic 5‐Methylene‐2‐furanone Release

Author(s) -

Roginskaya Marina,

Razskazovskiy Yuriy,

Bernhard William A.

Publication year - 2005

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200501956

Subject(s) - methylene , catalysis , decomposition , dna , x ray , chemistry , irradiation , high performance liquid chromatography , spermine , combinatorial chemistry , chromatography , biochemistry , organic chemistry , enzyme , physics , nuclear physics , quantum mechanics

Torn genes : DNA tetramers containing the 2‐deoxyribonolactone (dL) lesion have been isolated by HPLC from d(CGCG) and d(pCGCG) films irradiated with X‐rays. Upon treatment with spermine as a catalyst, the dL‐containing tetramers decompose to 5‐methylene‐2‐furanone (5‐MF; see scheme), a characteristic product of dL decomposition. Hence, 5‐MP can be used to quantify dL lesions in DNA.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research