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Chemical Primer Extension: Efficiently Determining Single Nucleotides in DNA
Author(s) -
Hagenbuch Patrizia,
Kervio Eric,
Hochgesand Annette,
Plutowski Ulrich,
Richert Clemens
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200501794
Subject(s) - nucleotide , primer extension , primer (cosmetics) , nucleobase , context (archaeology) , dna , chemistry , extension (predicate logic) , computational biology , combinatorial chemistry , stereochemistry , biochemistry , computer science , biology , organic chemistry , programming language , gene , paleontology
Rapid replication : Non‐enzymatic primer extension has previously been studied in the context of prebiotic chemistry, but not for practical applications. Reactions with primers featuring a 3′‐amino‐2′,3′‐dideoxynucleotide can be rapid and selective for all four templating nucleobases (see scheme). On a chip with immobilized capture strands, 500 fmol of template suffice for single‐nucleotide determinations within 2.7 h.