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A Method for Trapping Intermediates of Polyketide Biosynthesis with a Nonhydrolyzable Malonyl‐Coenzyme A Analogue
Author(s) -
Spiteller Dieter,
Waterman Claire L.,
Spencer Jonathan B.
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200501670
Subject(s) - polyketide , polyketide synthase , biosynthesis , chemistry , coenzyme a , stereochemistry , malonyl coa , enzyme , cofactor , biochemistry , reductase , beta oxidation
Trapped in the middle : To investigate the mechanistic details of polyketide biosynthesis, intermediates are trapped by a nonhydrolyzable malonyl‐coenzyme A analogue that reacts with a growing polyketide chain formed by a stilbene synthase. As the reverse transesterification reaction is not possible, the polyketide intermediates accumulate attached to the analogue (see scheme; Enz=enzyme, CoA=coenzyme A).