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Extension of the Applicable Range of Fluorescein: A Fluorescein‐Based Probe for Western Blot Analysis
Author(s) -
Kamiya Mako,
Urano Yasuteru,
Ebata Nobuyoshi,
Yamamoto Masami,
Kosuge Jyunichi,
Nagano Tetsuo
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200501542
Subject(s) - fluorescein , derivative (finance) , fluorescence , western blot , blot , chemistry , alkaline phosphatase , hydrolysis , biochemistry , physics , optics , enzyme , financial economics , economics , gene
The new star of Westerns : A highly sensitive fluorescence probe for alkaline phosphatase (ALP) based on a fluorescein derivative has been prepared and found to be suitable for use in Western blot analysis. The probe 1 is nonfluorescent but undergoes hydrolysis in the presence of ALP to yield a green‐fluorescent derivative 2 and may be used in multicolor labeling in combination with other probes.