Extension of the Applicable Range of Fluorescein: A Fluorescein‐Based Probe for Western Blot Analysis | Zendy

Kamiya Mako | Zendy; Urano Yasuteru | Zendy; Ebata Nobuyoshi | Zendy; Yamamoto Masami | Zendy; Kosuge Jyunichi | Zendy; Nagano Tetsuo | Zendy

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Extension of the Applicable Range of Fluorescein: A Fluorescein‐Based Probe for Western Blot Analysis

Author(s) -

Kamiya Mako,

Urano Yasuteru,

Ebata Nobuyoshi,

Yamamoto Masami,

Kosuge Jyunichi,

Nagano Tetsuo

Publication year - 2005

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200501542

Subject(s) - fluorescein , derivative (finance) , fluorescence , western blot , blot , chemistry , alkaline phosphatase , hydrolysis , biochemistry , physics , optics , enzyme , financial economics , economics , gene

The new star of Westerns : A highly sensitive fluorescence probe for alkaline phosphatase (ALP) based on a fluorescein derivative has been prepared and found to be suitable for use in Western blot analysis. The probe 1 is nonfluorescent but undergoes hydrolysis in the presence of ALP to yield a green‐fluorescent derivative 2 and may be used in multicolor labeling in combination with other probes.

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