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Genotyping—From Genomic DNA to Genotype in a Single Tube
Author(s) -
Strerath Michael,
Marx Andreas
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200501444
Subject(s) - genotyping , genomic dna , genotype , single nucleotide polymorphism , dna , biology , human genome , genetics , genome , computational biology , gene
Abstract Nucleotide variations in the human genome, such as single‐nucleotide polymorphisms, have been researched more intensively since it became apparent that these deviations are linked to various diseases and also several side effects of drugs. The investigation of genomic DNA in the laboratory requires routine methods that are time‐, labour‐, and cost‐effective. These criteria are fulfilled by so‐called closed‐tube methods, which are applied directly to isolated genomic DNA without any preamplification.