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A Functional Assay for Heparin in Serum Using a Designed Synthetic Receptor
Author(s) -
Wright Aaron T.,
Zhong Zhenlin,
Anslyn Eric V.
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200501437
Subject(s) - heparin , protamine , chemistry , phenylboronic acid , fluorescence , receptor , ammonium , dosing , selectivity , anticoagulant , pharmacology , combinatorial chemistry , chromatography , biochemistry , biology , medicine , organic chemistry , catalysis , physics , quantum mechanics
Excellent selectivity in highly competitive serum was displayed by a synthetic fluorescent receptor incorporating phenylboronic acid and ammonium groups for the therapeutic anticoagulant heparin. The concentration of heparin at clinically relevant dosing levels can be measured from the emission quenching. The binding is reversible as shown by the regeneration of the fluorescence on addition of protamine (see graph).