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Differential‐Frequency Saturation Transfer Difference NMR Spectroscopy Allows the Detection of Different Ligand–DNA Binding Modes
Author(s) -
Di Micco Simone,
Bassarello Carla,
Bifulco Giuseppe,
Riccio Raffaele,
GomezPaloma Luigi
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200501344
Subject(s) - saturation (graph theory) , dna , spectroscopy , differential (mechanical device) , minor groove , nuclear magnetic resonance spectroscopy , molecule , chemistry , nuclear magnetic resonance , biological system , computer science , analytical chemistry (journal) , physics , algorithm , mathematics , biology , quantum mechanics , biochemistry , combinatorics , thermodynamics , chromatography
Vive la différence! The binding of small molecules of pharmaceutical interest to DNA can be screened by differential‐frequency saturation transfer difference spectroscopy (DF‐STD). The technique allows the probing of DNA recognition phenomena, such as those of base‐pair intercalators, minor groove binders, and external backbone binders (see picture).