Premium
A Ligation and Photorelease Strategy for the Temporal and Spatial Control of Protein Function in Living Cells
Author(s) -
Pellois JeanPhilippe,
Muir Tom W.
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200501244
Subject(s) - ligation , function (biology) , chemistry , biophysics , nanotechnology , microbiology and biotechnology , biology , materials science
Design and light : A photocleavable synthetic moiety is ligated to the C terminus of a recombinant protein. In the ligated state, the chemical and protein components influence the properties of one another. Upon irradiation with light, both elements are released and their respective function is recovered. In this way a protein can be delivered into living cells and activated by UV irradiation at a desired time or location.