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Label‐Free Electronic Detection of Thrombin in Blood Serum by Using an Aptamer‐Based Sensor
Author(s) -
Xiao Yi,
Lubin Arica A.,
Heeger Alan J.,
Plaxco Kevin W.
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200500989
Subject(s) - aptamer , thrombin , methylene blue , chemistry , biosensor , dna , conformational change , biophysics , combinatorial chemistry , electron transfer , redox , electrochemistry , photochemistry , biochemistry , electrode , microbiology and biotechnology , inorganic chemistry , biology , platelet , photocatalysis , immunology , catalysis
Blue, gold, and DNA : A methylene blue (MB) tagged, thrombin‐binding DNA aptamer immobilized on a gold surface undergoes a large conformational change upon target binding (see schematic representation; eT: electron transfer). This folding produces a large, readily measurable change in redox current and allows the electrochemical detection of thrombin in blood serum.